RNA复制酶

现有可用的蛋白结构：
Pfam	结构 / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	结构概要

RNA dependent RNA polymerase
鉴定
标志	RdRP_1
Pfam	PF00680
Pfam宗系	CL0027
InterPro	IPR001205
SCOP	2jlg / SUPFAM
Pfam
现有可用的蛋白结构：
Pfam	结构 / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	结构概要

RNA复制酶; RNA-dependent RNA polymerase
	RNA复制酶的结构PDB 3PHU.
命名
系统命名
缩写
识别码
EC编号	2.7.7.48
CAS号	9026-28-2
数据库
IntEnz	IntEnz浏览
BRENDA	BRENDA入口
ExPASy	NiceZyme浏览
KEGG	KEGG入口
MetaCyc	代谢路径
PRIAM	概述
PDB	RCSB PDB PDBj PDBe PDBsum
基因本体	AmiGO / EGO
搜索
PMC	相关文献
PubMed	相关文献

RNA-directed RNA polymerase, flaviviral

鉴定

标志

RNA_pol_flaviviral

现有可用的蛋白结构：
Pfam	结构 / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	结构概要

RNA复制酶（RNA replicase）或名RNA依赖性RNA聚合酶（RNA-dependent RNA polymerase），缩写 RdRp 或 RDR，系一类能以RNA为模板复制RNA的酶。值得注意的是，这类酶与以DNA为模板合成RNA的RNA聚合酶（在转录中扮演重要角色的酶）不是同一类酶。

RNA复制酶对于那些反义 RNA病毒（例如流感病毒）的增殖来说，是一种至关重要的蛋白质^[2]^[3]。RNA复制酶能合成一条RNA单链的互补链^[4]^[5]。

结构

所有的RNA复制酶和不少RNA聚合酶都有一个折叠结构。这个折叠结构与三个亚结构域相连。上面三个结构域的形状和人的右手想像，因而分别被称为“四指”（fingers）、“手掌”（palm）、“拇指”（thumb）^[6]。其中，只有手掌亚结构域有一个由四条反向平行的多肽链构成的β-折叠结构和两个α-螺旋结构，在各类RNA复制酶中高度保守。其中，手掌亚结构域有三个高度保守的基元（A、B，以及C）。基元A（D-x(4,5)-D）和基元C（GDD）在空间上并列。这两个基元中的天冬氨酸残基与镁离子和锰离子相连。而B基元中的天冬酰胺残基则与在脱氧三磷酸核苷中选三磷酸核糖核苷的过程有关，上述过程可以确保合成的是RNA而不是DNA^[7]。RNA复制酶的结构域的组织形式保守^[8]，其催化中心也是一样。RNA复制酶的催化中心是由一系列包含保守的氨基酸残基的基元组成的，即使在同源性不高的编码序列下也保守。

参考

↑ Akutsu, M; Ye, Y; Virdee, S; Chin, JW; Komander, D. Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Proc. Natl. Acad. Sci. U.S.A. 2011-02, 108 (6): 2228–33. PMC 3038727 . PMID 21266548. doi:10.1073/pnas.1015287108.
↑ Koonin EV, Gorbalenya AE, Chumakov KM. Tentative identification of RNA-dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases. FEBS Lett. 1989-07, 252 (1–2): 42–6. PMID 2759231. doi:10.1016/0014-5793(89)80886-5.
↑ Zanotto PM, Gibbs MJ, Gould EA, Holmes EC. A reevaluation of the higher taxonomy of viruses based on RNA polymerases. J. Virol. 1996-09, 70 (9): 6083–96. PMC 190630 . PMID 8709232.
↑ Jin, Z; Leveque, V; Ma, H; Johnson, K. A.; Klumpp, K. Assembly, purification, and pre-steady-state kinetic analysis of active RNA-dependent RNA polymerase elongation complex. Journal of Biological Chemistry. 2012, 287 (13): 10674–83. PMC 3323022 . PMID 22303022. doi:10.1074/jbc.M111.325530.
↑ Kao CC, Singh P, Ecker DJ. De novo initiation of viral RNA-dependent RNA synthesis. Virology. 2001-09, 287 (2): 251–60. PMID 11531403. doi:10.1006/viro.2001.1039.
↑ Hansen JL, Long AM, Schultz SC. Structure of the RNA-dependent RNA polymerase of poliovirus. Structure. 1997-08, 5 (8): 1109–22. PMID 9309225. doi:10.1016/S0969-2126(97)00261-X.
↑ Gohara DW, Crotty S, Arnold JJ, Yoder JD, Andino R, Cameron CE. Poliovirus RNA-dependent RNA polymerase (3Dpol): structural, biochemical, and biological analysis of conserved structural motifs A and B. J. Biol. Chem. 2000-08, 275 (33): 25523–32. PMID 10827187. doi:10.1074/jbc.M002671200.
↑ O'Reilly EK, Kao CC. Analysis of RNA-dependent RNA polymerase structure and function as guided by known polymerase structures and computer predictions of secondary structure. Virology. 1998-12, 252 (2): 287–303. PMID 9878607. doi:10.1006/viro.1998.9463.

外部链接

[pmid21266548-1] Akutsu, M; Ye, Y; Virdee, S; Chin, JW; Komander, D. Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Proc. Natl. Acad. Sci. U.S.A. 2011-02, 108 (6): 2228–33. PMC 3038727 . PMID 21266548. doi:10.1073/pnas.1015287108.

[pmid2759231-2] Koonin EV, Gorbalenya AE, Chumakov KM. Tentative identification of RNA-dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases. FEBS Lett. 1989-07, 252 (1–2): 42–6. PMID 2759231. doi:10.1016/0014-5793(89)80886-5.

[pmid8709232-3] Zanotto PM, Gibbs MJ, Gould EA, Holmes EC. A reevaluation of the higher taxonomy of viruses based on RNA polymerases. J. Virol. 1996-09, 70 (9): 6083–96. PMC 190630 . PMID 8709232.

[4] Jin, Z; Leveque, V; Ma, H; Johnson, K. A.; Klumpp, K. Assembly, purification, and pre-steady-state kinetic analysis of active RNA-dependent RNA polymerase elongation complex. Journal of Biological Chemistry. 2012, 287 (13): 10674–83. PMC 3323022 . PMID 22303022. doi:10.1074/jbc.M111.325530.

[pmid11531403-5] Kao CC, Singh P, Ecker DJ. De novo initiation of viral RNA-dependent RNA synthesis. Virology. 2001-09, 287 (2): 251–60. PMID 11531403. doi:10.1006/viro.2001.1039.

[pmid9309225-6] Hansen JL, Long AM, Schultz SC. Structure of the RNA-dependent RNA polymerase of poliovirus. Structure. 1997-08, 5 (8): 1109–22. PMID 9309225. doi:10.1016/S0969-2126(97)00261-X.

[pmid10827187-7] Gohara DW, Crotty S, Arnold JJ, Yoder JD, Andino R, Cameron CE. Poliovirus RNA-dependent RNA polymerase (3Dpol): structural, biochemical, and biological analysis of conserved structural motifs A and B. J. Biol. Chem. 2000-08, 275 (33): 25523–32. PMID 10827187. doi:10.1074/jbc.M002671200.

[pmid9878607-8] O'Reilly EK, Kao CC. Analysis of RNA-dependent RNA polymerase structure and function as guided by known polymerase structures and computer predictions of secondary structure. Virology. 1998-12, 252 (2): 287–303. PMID 9878607. doi:10.1006/viro.1998.9463.

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